Identification of Virus

	Identification of Virus-Like Diseases Infecting Chayote by Symptomatology, Spectrophotometry and Transmission Electron Microscopy Julio S. Ligat - BSU Abstract Results of the study revealed that chayote leaf samples from artificially-inoculated had chlorotic spots and the naturally-infected had blotchy mosaic symptoms, which were associated with chayote mosaic virus, tymovirus. Purified virus preparations from the artificially inoculated and naturally infected samples had light scattering virus bands (about 2mm) that were seen on the 20% and 30% fractions of linear sucrose gradients. Spectrophotometrical analysis showed that the 30%fraction of linear sucrose gradient had the highest wavelength absorbance reading. Viewing of virus samples at 1:3 dilution in the Transmission Electron Microscope showed spherical densed particles at 30,000 print magnification. Back-inoculation tests of purified virus samples on Vigna umbellata showed localized necrotic and systemic chlorotic spots.
	Management of Clubroot in Crucifers Using Microbial Antagonist, Lime and PCNB Luciana M. Villanueva, Bernard S. Tad-awan, Alfonso O. Fagcayan - BSU Abstract The study was conducted at BSU Experimental Station, Balili, La Trindiad, Benguet to validate the efficacy of the three microbial antagonists against clubroot when applied alone or when integrated with lime and sub-lethal dose of Pentachloronitrobenzene (PCNB). Clubroot infection was significantly reduced when microbial antagonists were integrated with lime and sub-lethal dose of PCNB. Similarly, yield was significantly higher in plants applied with microbial antagonists than in the untreated control. Return on investment (ROI) was highest using Paecilomyces sp. alone followed by Pseudomonas sp. alone; lime; Flavobacterium sp. and sub-lethal dose of PCNB; and PCNB alone with 137.03; 124.26; 105.09; and 101.07%, respectively. The use of such integration is cost-effective, easy to mass produce, and safe to apply. This technology will prevent the development of resistance when rotated in control program with compatible chemicals.
	*Coir Dust Combined with Rooting Hormones Enhance Rooting of Coffea arabicaCuttings* *Avelina M. Galacio, Josephine B. Ayban - BPI* Abstract The study was conducted from February to April 2002 which aimed to evaluate the effectiveness of different rooting hormones and rooting media for arabica cuttings and identify most effective rooting hormone and the best rooting medium for arabica cuttings. Hormex solution at the rate of one drop per 250 ml water combined with coir dust as rooting medium enhanced early rooting cuttings at 19 days. Moreover, the same treatment initiated the most number and longest roots.
	*Effect of Different Growth Regulators and Germination Techniques in Breaking Dormancy of Coffea arabica* Seeds Avelina M. Galacio, Josephine B. Ayban - BPI Abstract** The study was conducted from January to March, 2002. It aimed to determine the growth regulators suitable for breaking dormancy of arabica seeds, evaluate and identify the most effective germination technique for arabica seeds. Coffee seeds without seed coat treated with calcium carbide (carboro) at the rate of 10 grams/kg seeds for 15 days germinated earliest at 22 days while other treatments germinated late at the range of 35 to 60 days. Further, calcium carbide treatment produced the highest average number and length of roots compared to the other treatments.